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Objective To investigate the results of MRI and electroencephalogram examination and pathological examination of drug refractory epilepsy. Methods 70 cases with drug refractory epilepsy were treated with systemic antiepileptic drugs, but they still could not control their condition, and they were given MRI, EEG and pathological examination respectively. MRI, EEG and pathological findings of 70 cases were recorded, and the conclusion was drawn after statistical analysis. Results Compared with pathological examination, the localization accuracy of MRI for epileptogenic foci and related lesions was 88.57%, the accuracy of localization of EEG for epileptogenic foci and related disease was 51.43%, the difference was statistically significant (P<0.05); MRI examination of the epileptogenic lesions and qualitative accuracy the rate of up to 75.71%. Conclusion According to the patients with drug intractable epilepsy, the coincidence rate of MRI examination and pathological examination is higher. Compared with EEG, it can provide clinicians with more reliable basis for the diagnosis and treatment of drug refractory epilepsy. It is helpful to protect the curative effect of these patients.
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Objective To investigate the results of MRI and electroencephalogram examination and pathological examination of drug refractory epilepsy. Methods 70 cases with drug refractory epilepsy were treated with systemic antiepileptic drugs, but they still could not control their condition, and they were given MRI, EEG and pathological examination respectively. MRI, EEG and pathological findings of 70 cases were recorded, and the conclusion was drawn after statistical analysis. Results Compared with pathological examination, the localization accuracy of MRI for epileptogenic foci and related lesions was 88.57%, the accuracy of localization of EEG for epileptogenic foci and related disease was 51.43%, the difference was statistically significant (P<0.05); MRI examination of the epileptogenic lesions and qualitative accuracy the rate of up to 75.71%. Conclusion According to the patients with drug intractable epilepsy, the coincidence rate of MRI examination and pathological examination is higher. Compared with EEG, it can provide clinicians with more reliable basis for the diagnosis and treatment of drug refractory epilepsy. It is helpful to protect the curative effect of these patients.
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The medical aid policy aimed at reducing medical expense of the poor group,which could not solve impoverished problem effectively.People who lack health ability could not get out of poverty ultimately.Health poverty became the direct reason for low income of residents,which blocked the realization of their rights to health and increased the difficulties to complete the objective of helping the poor by 2020.Medical aid policy was the last line of defense in social security system,therefore,policies to relieve health poverty could combine with medical aid policy.Given this,the destination of medical aid system should tend to combine reducing medical expense and improving health ability of poor people.To ensure the realization of citizen's right to health and reduce illness-related poverty,it needed to improve the system and public health service system,emphasize health education and promote social organization participation.
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Chemotaxis is the response ability of motile cells to chemicals gradients in environment and the migration toward higher concentration of chemoattractant or lower concentration of repellent. This mechanism is a basic nature of microorganisms to adapt to the environmental changes. The research of microbial chemotaxis is of great significance in utilizing bacteria to solve environment problems, control the pathogen infection, and develop microbial industrial projects. Microfluidic devices can realize qualitatively and quantitatively detect of bacterial chemotaxis. In comparison with traditional detect methods, microfluidic assay has an accurate control over bacterial microenvironment, with a higher sensitivity. In the past few years, bacterial chemotaxis study based on microfluidic assay was developed rapidly. In this paper, the microfluidic chemotaxis detectors that appeared in recent years were introduced from the aspect of chip structure, working principle and their applications. Finally, we provided insights into the challenges of bacterial chemotaxis and provided future perspectives.
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Objective To detect the level of S100-β protein in the serum of patients with closed craniocerebral injury and analyze its clinical value to assess the diagnosis and prognosis of the disease.Methods The expression of quantitative analysis method to detect 31 cases of healthy control group,40 cases of patients with severe craniocerebral injury and 34 cases of craniocerebral injury patients on admission S100-β protein level by the receiver operating characteristic curve (ROC) analysis,to explore the correlation between the prognosis of GOS,closed craniocerebral injury diagnosis efficacy.Results In the control group,mild and severe closed craniocerebral injury group S100-β protein levels were 0.137 ±0.025,0.192 ± 0.038 and 0.276 ±0.097 ng/ml,respectively.Compared with the control group (F=0.126,P=0.008),light and heavy closed craniocerebral injury group (F=38.17,P=0.001) of serum S100-β protein levels were significantly increased,the difference was statistically significant (P＜0.01).There were significant differences in the level of serum S100-β light and heavy craniocerebral injury group (P＜0.05).Serum S100-βprotein differential control group and brain injury group AUC 0.870 (95％ CI:0.776 ～ 0.964,P＜ 0.01).S100-β protein identification in healthy control group with severe craniocerebral injury group AUC was 0.914 (95 ％ CI:0.850～0.978,P＜ 0.01).The score was negatively correlated with serum S100-β protein level and the prognosis of craniocerebral injury in GOS (r=-0.792,P＜0.01).Conclusion S100-β protein significantly increased in serum of light and heavy closed craniocerebral injury patients,and negatively correlated with the GOS score of patients,can be used for the auxiliary diagnosis and prognosis of craniocerebral injury.
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AIM:To investigate the role of microRNA-101-3p (miRNA-101-3p) on the proliferation, apopto-sis and invasion of gastric cancer cells and the possible regulatory mechanisms .METHODS: The expression of miRNA-101-3p in two kinds of gastric cancer cells and a gastric mucosal cell line was detected by real -time PCR.The miRNA-101-3p was overexpressed by Lipofectamine 2000 transfection with miRNA-101-3p mimics.The effects of miRNA-101-3p on cell cycle distribution and apoptosis were analyzed by flow cytometry .The effects of miRNA-101-3p on cell proliferation and migration abilities were detected by CCK-8 assay, trypan blue exclusion test and Transwell assay .The protein expression of enhancer of zeste homolog 2 ( EZH2) was determined by Western blot .RESULTS: The expression of miRNA-101-3p in gastric cancer cells was lower than that in gastric mucosal cells (P<0.05).The gastric cancer cell MGC-803 had the low-est expression level of miRNA-101-3p.The result of flow cytometry showed that the population of S phase was reduced , and the population of G0/G1 phase and the early stage apoptotic rate were increased after the expression of miRNA-101-3p was overexpressed (P<0.05).The results of CCK-8 assay, trypan blue exclusion test and Transwell assay showed that overex-pression of miRNA-101-3p significantly reduced the proliferation and migration abilities of gastric cancer cells (P<0.05). Overexpression of miRNA-101-3p decreased the protein level of EZH2 (P<0.05).CONCLUSION:miRNA-101-3p may suppresses the gastric cancer cell proliferation and migration , and promotes the gastric cancer cell apotosis by down-regula-tion of EZH2.